ADC Practice Question — Disinfection Methods

How many consecutive cycles must be run with biological indicators during validation?

Infection control in the ADC exam tests decision-making around exposure incidents, instrument classification, and decontamination protocols. This exam-level vignette tests your ability to work through clinical details and arrive at the correct management.

Dr Park's practice has purchased a new Type B steam steriliser to replace their aging unit. The steriliser service technician is scheduled to perform initial validation. The practice manager wants to understand the validation process and asks Dr Park about biological indicator requirements. How many consecutive cycles must be run with biological indicators during validation?

A. One cycle with multiple BIs
B. Two cycles in triplicate
C. Three consecutive cycles with test packs in triplicate, plus one unsterilised positive control
D. Five cycles with one BI each

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Sources cited

Therapeutic Guidelines v4 Odell's 3rd & 4th Ed IADT 2020 Code of Conduct CMS (Evans) Infection Control + more

Show the answer and cited explanation

Correct answer: C

Validation requires at least two BIs per small steriliser chamber, placed at the cold spot (typically bottom of chamber). Test packs are prepared in triplicate for three consecutive cycles, plus one unsterilised positive control. All are incubated at 55-56°C for 24-48 hours. (ICG p.57)

Source: ICG Section D: Biological Indicators & Validation

Remy's study tip — The differentiator is usually the Spaulding classification level or the step order in a protocol.

Exam preparation FAQ

How should I prepare for Infection Control in the ADC exam?

Build competence across all topics. The exam tests application of guidelines to clinical scenarios, not memorisation.

How do I approach an unfamiliar Disinfection Methods vignette?

Extract key clinical data from the stem before reading options. There are usually 3–4 critical variables that determine the correct answer.

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